Full text is available at the source.
Target-specific mutations efficiency at multiple loci of CRISPR/Cas9 system using one sgRNA in soybean
Efficiency of CRISPR/Cas9 gene editing at multiple sites in soybean using one guide RNA
AI simplified
Abstract
Mutation frequencies of 64.71% for GmPDS, 60.0% for GmFAD2, and 42.86% for GmALS were achieved using a single sgRNA CRISPR/Cas9 system in soybean.
- The CRISPR/Cas9 system was optimized to assess mutation efficiency in soybean's multiple-copy genes.
- Simultaneous site-directed mutagenesis was successfully performed at two homoeologous loci with one sgRNA.
- Chimeric and heterozygous mutations were the most common outcomes observed.
- Phenotypic associations were identified with mutation patterns at specific target loci.
- The findings indicate that while effective, the system exhibits relatively low transformation efficiency.
AI simplified