Expression of Transcription Factor 21 (TCF21) and Upregulation Its Level Inhibits Invasion and Metastasis in Esophageal Squamous Cell Carcinoma

Jun 18, 2018Medical science monitor : international medical journal of experimental and clinical research

Higher Levels of TCF21 Protein Reduce Spread and Invasion in Esophageal Squamous Cell Cancer

AI simplified

Circadian Biology on OpenScience ↗PubMed ↗DOI ↗

Abstract

TCF21 mRNA and protein expression were both downregulated in esophageal cancer tissues compared with adjacent normal tissues.

Low expression of TCF21 is closely correlated with N stage in esophageal squamous cell carcinoma (ESCC).
Kaplan-Meier survival analysis indicates that patients with lower TCF21 expression have poorer prognosis.
Overexpression of TCF21 significantly inhibits proliferation, migration, and invasion in esophageal cancer cell lines.
Upregulation of TCF21 is associated with increased expression of the tumor suppressor Kiss-1.
TCF21 may reverse epithelial-mesenchymal transition (EMT) by modulating the expression of related proteins.

AI simplified

BACKGROUND Transcription factor 21 (TCF21), a member of the class A of basic helix-loop-helix family, has been widely identified as a tumor suppressor. Growing evidence has demonstrated the downregulation of TCF21 in distinct cancers. The aim of this study was to explore the expression and biological functions of TCF21 in esophageal squamous cell carcinoma (ESCC). MATERIAL AND METHODS TCF21 expression in esophageal cancer cell lines and carcinomas tissues were detected, and its associations with clinical characteristics were analyzed. We carried out this study of biological functions and underlying mechanisms using TE10 and KYSE510 cell lines. RESULTS TCF21 mRNA and protein expression were both downregulated in esophageal cancer tissues compared with adjacent normal tissues. Low expression of TCF21 was closely correlated with N stage. In Kaplan-Meier survival analysis, patients with lower TCF21 expression had poorer prognosis. Overexpression of TCF21 greatly inhibited the proliferation, migration, and invasion in both TE10 and KYSE510 cell lines. Furthermore, mechanistic studies showed that with TCF21 gene overexpressed, the expression of tumor suppressor Kiss-1 was upregulated and epithelial-mesenchymal transition (EMT) related proteins (E-cadherin, N-cadherin, Snail, Twist, and Vimentin) which participate in cancer cell invasion and metastasis, were reversed. CONCLUSIONS TCF21 is downregulated in ESCC, and its low expression is closely correlated with N stage and predicts a poor prognosis. TCF21 functions as a tumor suppressor in ESCC progression, and enhancement of its expression levels may be partly through promoting Kiss-1 expression to reverse EMT by modulating EMT-related gene expression. Thus, TCF21 can potentially be used as a treatment target for ESCC.

Full Text

We can’t show the full text here under this license. Use the link below to read it at the source.

View full text ↗

Expression of Transcription Factor 21 (TCF21) and Upregulation Its Level Inhibits Invasion and Metastasis in Esophageal Squamous Cell Carcinoma

Abstract

Full Text

You found one interesting study. We’ll send the next 7.

what lands in your inbox each week:

Recent issues from the circadian biology brief

Abstract

Full Text

Related papers

You found one interesting study. We’ll send the next 7.

what lands in your inbox each week:

Recent issues from the circadian biology brief