Full text is available at the source.
Abstract
Therapeutic messenger RNAs (mRNAs) could enhance treatment options for various diseases by optimizing their stability and efficiency.
- Advancements in secondary structure optimization may improve mRNA performance in therapeutics.
- Artificial intelligence tools like RNAdegformer and LinearDesign are being used to balance structural stability and translational output.
- The roles of untranslated regions at both ends of the mRNA are crucial for ribosome recruitment and controlling mRNA decay.
- Chemical modifications, such as pseudouridine substitution, may increase mRNA stability and lower immune reactions.
- Circular RNA is emerging as a method to extend the longevity of transcripts.
- Various delivery technologies, including lipid nanoparticles and extracellular vesicles, could enhance mRNA protection and cellular uptake.
Simplified