A precise automated system for controlling temperature in studies of circadian rhythms outside living organisms.

ThermoClock, an Arduino-based temperature regulation system, can reach target temperatures within five minutes after a setpoint change.

• The system offers precise, automated temperature control for circadian rhythm research in ex vivo and in vitro studies.
• It is built with off-the-shelf components and open-source software, costing approximately $450 and requiring under 10 hours to assemble.
• ThermoClock's modular design allows simultaneous control of multiple temperature conditions, potentially reducing manual intervention and user error.
• Each module utilizes a Proportional-Integral-Derivative (PID) controller for real-time temperature adjustments.
• ThermoClock can operate up to five temperature modules, enhancing experimental versatility and throughput.
• It demonstrates significantly faster heating and cooling (< 0.001) compared to traditional programmable incubators.

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