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Abstract
Evidence indicates that translesion synthesis polymerases can preferentially insert specific nucleotides opposite apurinic/apyrimidinic sites.
- Base editors create defined DNA lesions that require cellular repair pathways to process.
- Apurinic/apyrimidinic sites serve as a critical point where the repair pathway choice influences the outcome.
- Product heterogeneity has been viewed as a limitation of base editing.
- Competition among repair polymerases significantly affects the editing outcome.
- Engineering the recruitment of specific polymerases may allow for more predictable nucleotide incorporation.
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