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Abstract
A detection limit of 0.17 copies/μL was achieved for the H1N1 influenza virus using a novel biosensor.
- The biosensor utilizes a 3D spherical DNA nanostructure combined with CRISPR/Cas12a for enhanced detection capabilities.
- Binding of the H1N1 virus to specific nucleic acid aptamers triggers a change that activates the Cas12a enzyme, leading to signal amplification.
- Spike recovery rates in various matrices, including chicken serum and milk, ranged from 91.89% to 104.03%, indicating reliable performance.
- The total detection time is reduced to 40 minutes, which is three times faster than traditional qPCR methods.
- The structural design of the 3D nanostructure contributes to its stability during storage, which was an unexpected finding.
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