Ultrasensitive detection of lead ion in tea samples using a versatile and robust multi-DNAzyme DNA machine mediated CRISPR/Cas12a signal amplification system

The detection platform achieves a detection limit of 67.53 pM for lead ion contamination in tea.

• The platform integrates a multifunctional DNAzyme machine with the CRISPR/Cas12a system for enhanced detection.
• Activation of the DNA machine leads to multiple cycles that generate a poly-A sequence, which then activates Cas12a.
• This activation triggers trans-cleavage of fluorescent probes, resulting in significant signal amplification.
• The assay demonstrates a wide linear range of 0.01-100 nM and high recovery rates above 96% in real tea samples.
• The approach simplifies assay design by eliminating the need for auxiliary probes while maintaining specificity.

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