Uridine dynamic administration affects circadian variations in lipid metabolisms in the liver of high-fat-diet-fed mice

Jul 13, 2019Chronobiology international

Uridine treatment changes daily patterns of fat metabolism in the liver of high-fat-fed mice

AI simplified

Circadian Biology on OpenScience ↗PubMed ↗DOI ↗

Abstract

Uridine supplementation significantly reduced body weight gain in mice fed a high-fat diet (p < .05).

Liver weight and ratio demonstrated a strong dependence on the time of uridine administration (p < .001).
The timing of uridine supplementation influenced the expression of genes related to uridine metabolism.
Uridine affected the levels of 10 fatty acids and various glucose and lipid metabolism genes based on the timing of administration (p < .05).
Overall, uridine supplementation may alter diurnal variations in liver metabolism, contributing to weight loss in high-fat diet-fed mice.

AI simplified

Carbohydrate and lipid metabolism in the liver has been reported to follow a certain circadian rhythm. Moreover, uridine supplementation also affected glucose and lipid homeostasis in previous studies; however, the mechanisms remain unclear. Therefore, this study was conducted to investigate whether uridine supplementation at an appropriate time during the day can alleviate obesity in mice. C57BL/6J mice were randomly assigned to four groups (n = 24 per group) with different diets for 2 weeks: (1) HFD group, fed a diet containing 60% fat calories; (2) DUR group, fed with HFD supplemented with 400 mg/kg uridine during zeitgeber time (ZT0) 0 - zeitgeber time 12 ZT12; (3) NUR group, fed HFD supplemented with 400 mg/kg uridine during ZT12 - ZT24. Starting at ZT4, liver samples were collected every 6 h for 24 h. Results showed that uridine supplementation, independent of the time of administration during the day, significantly reduced body weight gain (< .05). Furthermore, liver weight and ratio showed a strong time dependence (< .001). Additionally, oral administration of uridine during daytime or nighttime changed the expression levels of genes involved in the metabolism of uridine (SLC29A1, UMPS, UPP, UGT1A1, and DHODH;< .05). Furthermore, uridine affected the levels of 10 fatty acids, lipid and glucose gene (FASN, LCAT, PC, PEPCK, GSK3β, and GLUT2;< .05) depending on the timing of administration (< .05). In conclusion, oral supplementation with uridine affected the diurnal variations in liver nucleotide and lipid metabolism, which contributed to the weight loss in HFD-fed mice. P P P P P

Full Text

Full text is available at the source.

View full text ↗

Uridine dynamic administration affects circadian variations in lipid metabolisms in the liver of high-fat-diet-fed mice

Abstract

Full Text

You found one interesting study. We’ll send the next 7.

what lands in your inbox each week:

Recent issues from the circadian biology brief

Abstract

Full Text

Related papers

You found one interesting study. We’ll send the next 7.

what lands in your inbox each week:

Recent issues from the circadian biology brief