The Journal of pharmacy and pharmacologyMar 5, 2020

Urine metabolite changes linked to Huangqi Jianzhong Tang treatment in rats with chronic stomach lining loss

Circadian Biology Weekly Brief ↗PubMed ↗DOI ↗

Updated Apr 3, 2026

Abstract

Thirty-two urine metabolites were significantly regulated by Huangqi Jianzhong Tang (HQJZ) treatment in chronic atrophic gastritis (CAG) rats.

Nineteen and 16 potential biomarkers related to CAG were identified through nuclear magnetic resonance and mass spectrometry, respectively.
The regulatory effect of HQJZ on urinary metabolism disorders was comparable to the positive drug teprenone.
Three metabolic pathways—valine, leucine and isoleucine metabolism, TCA cycle, and glycine, serine and threonine metabolism—were found to be most relevant to HQJZ treatment.
The main mechanism of HQJZ may involve energy balance, inflammatory inhibition, immune system improvement, and reduction of oxidative stress.

Simplified

OBJECTIVES: As a traditional Chinese medicine (TCM), Huangqi Jianzhong Tang (HQJZ) has a good efficacy in treating chronic atrophic gastritis (CAG). Our objective was to determine its mechanism based on the urine comprehensive metabolome.

METHODS: In the study, a metabolomic approach was applied to reveal the efficacy of HQJZ on the constructed CAG rats coupled with proton nuclear magnetic resonance (H NMR) and ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF MS). 1

KEY FINDINGS: The results showed the regulatory effect of HQJZ on urinary metabolism disorder in CAG rats was similar to the positive drug teprenone. Nineteen and 16 potential biomarkers related to CAG were detected by NMR and UPLC-Q/TOF MS, respectively. Thirty-two urine metabolites were significantly regulated by HQJZ treatment. Combined with MetPA and partial least square regression analysis (PLS-RA), three metabolic pathways of valine, leucine and isoleucine, TCA cycle, and glycine, serine and threonine metabolism were the most relevant pathways for HQJZ treatment.

CONCLUSIONS: The main mechanism of HQJZ might be due to the balance of energy consumption, inflammatory inhibition, improvement of the immune system and oxidative stress on the constructed CAG rats. These findings provided comprehensive metabolic information of TCM by parallel measurements by LC-MS and NMR.

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Urine metabolite changes linked to Huangqi Jianzhong Tang treatment in rats with chronic stomach lining loss

Abstract

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