Wuzi Yanzong Pill relieves MPTP-induced motor dysfunction and neuron loss by inhibiting NLRP3 inflammasome-mediated neuroinflammation

Jul 20, 2023Metabolic brain disease

Wuzi Yanzong Pill may reduce movement problems and nerve cell loss by blocking inflammation in a Parkinson’s disease model

AI simplified

Gut-Brain Axis on OpenScience ↗PubMed ↗DOI ↗

Abstract

Oral administration of Wuzi Yanzong Pill (WYP) in MPTP-induced Parkinson disease mice for 2 weeks ameliorated abnormal motor dysfunction.

WYP treatment reduced the loss of dopaminergic neurons in the substantia nigra pars compacta.
The intervention inhibited microglial activation in both MPTP-induced PD mice and LPS-stimulated microglia cells.
WYP decreased the expression of several inflammatory markers, including IL-6, TNF-α, and IL-1β.
An increase in IL-10 expression was observed in the substantia nigra of treated PD mice.
WYP treatment was associated with the inhibition of the NLRP3 inflammasome and the TLR4/MyD88/NF-κB signaling pathway.
Further investigation is needed to clarify the exact mechanism of WYP's action on these pathways.

AI simplified

Parkinson disease (PD) is an age-related neurodegenerative disease, which is associated with the loss of dopaminergic neurons (DA neurons) in the substantia nigra pars compacta (SNpc), and neuroinflammation may lead to the occurrence of PD. Wuzi Yanzong Pill (WYP) has demonstrated neuroprotective and anti-inflammatory properties, but its molecular mechanism of action is still unclear. In this study, we used 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD mice and LPS-mediated BV2 microglia to explore WYP intervention, anti-inflammatory effect and molecular mechanism in vivo and in vitro. The results showed that oral administration of WYP in MPTP-induced PD mice for 2 weeks ameliorated abnormal motor dysfunction, attenuated the loss of TH + neurons in SNpc, protected dopaminergic neurons, and inhibited the activation of microglia in MPTP-induced PD mice and LPS-stimulated BV2 cell. Meanwhile, WYP intervention inhibited the expression of IL-6, TNF-α, Pro-IL-1β, IL-1β, Pro-IL-18, IL-18 and enhanced the expression of IL-10 in the SNpc of PD mice. Simultaneously, WYP intervention inhibited the expression of NLRP3 inflammasome, accompanied by the decrease of the TLR4/MyD88/NF-κB pathway. However, the exact target and interaction of WYP on NLRP3 inflammasome and TLR4/MyD88/NF-κB pathway still needs to be further investigated.

Full Text

Full text is available at the source.

View full text (PDF) ↗View full text (HTML) ↗

Wuzi Yanzong Pill relieves MPTP-induced motor dysfunction and neuron loss by inhibiting NLRP3 inflammasome-mediated neuroinflammation

Abstract

Full Text

You found one interesting study. We’ll send the next 7.

what lands in your inbox each week:

Recent issues from the gut-brain axis brief

Abstract

Full Text

Related papers

You found one interesting study. We’ll send the next 7.

what lands in your inbox each week:

Recent issues from the gut-brain axis brief