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A reverse transcription-free CRISPR/Cas12a biosensor for ultrasensitive detection of SARS-CoV-2 variants
A CRISPR-based sensor for ultra-sensitive detection of SARS-CoV-2 variants without reverse transcription
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Abstract
This method enables the detection of SARS-CoV-2 mutations within 1 hour, achieving a detection limit of 7.49 aM.
- The biosensor detects both single- and double-stranded amplification products of SARS-CoV-2 mutant variants.
- It generates double-stranded DNA products with PAM sequences and single-stranded DNA products without PAM sequences.
- Both types of amplification products can be recognized by the crRNA, enhancing detection sensitivity.
- The assay shows 100% sensitivity and 100% specificity in detecting SARS-CoV-2 mutations in 106 clinical samples compared to DNA sequencing.
- High specificity is observed in differentiating mutant variants from wild-type sequences.
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