Eco-Friendly Zinc Oxide Nanoparticles Made from Marshmallow Flower Extract and Coated with Chitosan May Help Heal Diabetic Wounds by Reducing Inflammation Signals
(ZnO-NPs) synthesized from Althaea officinalis demonstrated a mean size of 76 nm and showed significant antibacterial properties.
The green-synthesized ZnO-NPs displayed a peak absorbance at 390 nm during UV-visible spectrum analysis.
Energy-dispersive X-ray spectrometry confirmed the presence of zinc and oxygen in the nanoparticles.
The antibacterial efficacy of the formulation was assessed, revealing notable inhibition against both Gram-positive and Gram-negative bacteria.
In in-silico studies, major components showed a potential binding affinity to targets associated with wound healing.
The A.O.-ZnO-NPs formulation resulted in decreased levels of inflammatory markers IL-6, IL-1β, and TNF-α, while increasing IL-10 levels.
Histopathological analysis indicated a reduction in inflammation and improved wound healing in rat models treated with the nanoparticles.
Simplified
BACKGROUND: Diabetes Mellitus is a multisystem chronic pandemic, wound inflammation, and healing are still major issues for diabetic patients who may suffer from ulcers, gangrene, and other wounds from uncontrolled chronic hyperglycemia.contain bioactive compounds such as flavonoids and phenolics that support wound healing via antioxidant, anti-inflammatory, and antibacterial properties. Our study aimed to develop a combination of eco-friendly formulations of green synthesis of ZnO-NPs byextract and further incorporate them into 2% (CS) gel. Marshmallows or Althaea officinalis (A.O.) Althaea officinalis
METHOD AND RESULTS: First, develop eco-friendly green (ZnO-NPs) and incorporate them into a 2% chitosan (CS) gel. In-vitro study performed by UV-visible spectrum analysis showed a sharp peak at 390 nm, and Energy-dispersive X-ray (EDX) spectrometry showed a peak of zinc and oxygen. Besides, Fourier transforms infrared (FTIR) was used to qualitatively validate biosynthesized ZnO-NPs, and transmission electron microscope (TEM) showed spherical nanoparticles with mean sizes of 76 nm and Zeta potential +30mV. The antibacterial potential of A.O.-ZnO-NPs-Cs was examined by the diffusion agar method against Gram-positive (and) and Gram-negative bacteria (and). Based on the zone of inhibition and minimal inhibitory indices (MIC). In addition, an in-silico study investigated the binding affinity of. major components to the expected biological targets that may aid wound healing., A.O-ZnO-NPs group showed reduced downregulation of IL-6, IL-1β, and TNF-α and increased IL-10 levels compared to the control group signaling pathway expression levels confirming the improved anti-inflammatory effect of the self-assembly method.and histopathological analysis revealed the superiority of the nanoparticles in reducing signs of inflammation and wound incision in rat models. Staphylococcus aureus Bacillus subtilis Escherichia coli Pseudomonas aeruginosa A.O Althaea Officinalis In-vivo study
CONCLUSION: These biocompatible green zinc oxide nanoparticles, by usingchitosan gel ensure an excellent new therapeutic approach for quickening diabetic wound healing. Althaea Officinalis
Key numbers
98.1%
Wound Contraction Percentage Increase
Wound contraction percentage in group treated with ZnO-NPs gel.
Reduced downregulation of IL-6
IL-6 Level Decrease
Comparison of IL-6 levels in treated vs. control groups.
22.51 mm
Diameter of Zone of Inhibition
Zone of inhibition observed in antibacterial susceptibility testing.
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