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20-Hydroxyecdysone (20E) Primary Response Gene E93 Modulates 20E Signaling to Promote Bombyx Larval-Pupal Metamorphosis
Gene E93 controls 20-hydroxyecdysone hormone signals to help silkworms change from larvae to pupae
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Abstract
E93 expression in the fat body is markedly low prior to the wandering stage and abundant during larval-pupal metamorphosis.
- E93 expression is induced by 20-hydroxyecdysone (20E) and suppressed by juvenile hormone (JH) across multiple silkworm alleles.
- Reducing E93 expression through RNA interference disrupts 20E signaling and affects processes like autophagy and cell dissociation.
- Lower E93 levels lead to decreased expression of pupal-specific cuticle protein genes and hinder growth and differentiation of wing discs.
- The two HTH domains in E93 are essential for activating a subset of genes responsive to 20E, while certain motifs inhibit its activity.
- E93 forms a physical association with the EcR-USP complex, which is enhanced by 20E, affecting its transcriptional function.
- E93 binds to specific DNA motifs in the Atg1 promoter region to induce gene expression related to metamorphosis.
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