Developmental Regulation of Glycolysis by 20-hydroxyecdysone and Juvenile Hormone in Fat Body Tissues of the Silkworm, Bombyx mori

Aug 24, 2010Journal of molecular cell biology

Hormone control of sugar breakdown during development in silkworm fat storage tissues

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Abstract

Many genes associated with energy metabolism were down-regulated during molting and pupation when 20-hydroxyecdysone (20E) levels are high.

20E treatment decreased both mRNA levels and activities of key enzymes in the glycolytic pathway.
RNA interference targeting the 20E receptor EcR-USP resulted in increased glycolytic enzyme activity, opposing the effects of 20E.
Juvenile hormone (JH) treatment increased mRNA levels and activities of glycolytic enzymes, likely counteracting the effects of 20E.
20E may function as a general inhibitor of glycolysis in the fat body of Bombyx mori during critical developmental stages.

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20-Hydroxyecdysone (20E) and juvenile hormone (JH) control a variety of physiological events during insect development and metamorphosis. To understand how 20E and JH developmentally regulate energy metabolism in insects, we performed a genome-wide microarray analysis of fat body tissues isolated from the silkworm, Bombyx mori. Many genes involved in energy metabolism, including genes in the glycolytic pathway, were down-regulated during molting and pupation, when 20E levels are high. Notably, 20E treatment exhibited inhibitory effects on key glycolytic enzyme mRNA levels and activities, and RNA interference of the 20E receptor EcR-USP had the opposite effects to 20E treatment. Meanwhile, JH treatment stimulated both mRNA levels and activities of the key glycolytic enzymes, presumably via antagonizing the 20E action. Taken together, we conclude that 20E acts as a general blocker for glycolysis in the Bombyx fat body during molting and pupation, whereas the physiological role of JH is contrast with 20E during molting.

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Developmental Regulation of Glycolysis by 20-hydroxyecdysone and Juvenile Hormone in Fat Body Tissues of the Silkworm, Bombyx mori

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