3D Printing of genipin crosslinked alginate/gelatin Ink for tissue engineering application

The bioink achieved a compressive modulus of up to 0.023 MPa at 1 % genipin concentration.

• A novel 3D-printable bioink was created using a composite of 7 % gelatin and 9 % alginate.
• Genipin crosslinking improved compressive strength and degradation resistance of the bioink.
• Biological tests showed cell viability exceeding 100 % and good cell adhesion at 0.1 % and 0.5 % genipin concentrations.
• Higher genipin concentrations (1 %) led to decreased cell adhesion and viability, dropping to 81 %.
• The study suggests that these hydrogels have tunable properties, making them suitable for various tissue engineering applications.

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