Improved adenine base editor that works with fewer DNA restrictions, edits better, and causes fewer unintended changes

47% of pathogenic point mutations may be corrected by ABE-induced A·T-to-G·C conversions.

• A new adenine base editor, CE-8e-SpRY, shows improved editing activity at NRN PAMs compared to NYN PAMs.
• CE-8e-SpRY is capable of targeting nearly all genomic sites and exhibits the highest targeting efficiency among tested SpRY-based adenine base editors.
• This editor demonstrates reduced off-targeting effects in both RNA and DNA.
• With optimized guide RNAs, CE-8e-SpRY can achieve efficient editing at specific disease-related sites that conventional adenine base editors cannot.

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