A novel base editor SpRY-ABE8eF148A mediates efficient A-to-G base editing with a reduced off-target effect

Jan 9, 2023Molecular therapy. Nucleic acids

A new base editor efficiently changes A to G DNA letters with fewer unintended edits

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Abstract

SpRY-ABE8e increases A-to-G editing efficiency while decreasing RNA off-target effects.

  • Adenine base editors can create specific mutations, useful for correcting certain genetic variants.
  • The new editor, SpRY-ABE8e, narrows the editing range and enhances efficiency for specific target sequences.
  • SpRY-ABE8e shows improved precision for addressing G·C-to-T·A pathogenic variants compared to its predecessor.
  • A new bioinformatics tool was created to support the development of precise adenine base editors.

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