Agrobacterium-Mediated Genetic Transformation and Genome Editing Using CRISPR-Cas9 Constructs in Rice

Oct 1, 2025Methods in molecular biology (Clifton, N.J.)

Using Agrobacterium and CRISPR-Cas9 to Genetically Modify and Edit Rice

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Abstract

High transformation efficiency can be achieved in recalcitrant rice genotypes using CRISPR-Cas9.

CRISPR-Cas9 is an essential tool for creating precise genetic changes in plants.
Recalcitrant rice genotypes typically show poor response to traditional genetic transformation methods.
This protocol provides a detailed procedure to improve genetic transformation in these challenging rice varieties.
The method is designed to expedite the genome editing process in rice.

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Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas)9 has become an essential tool in every molecular plant breeding laboratory. CRISPR-Cas9 causes highly precise knock-out mutations in plants that can be exploited in crop improvement programmes. However, poor response to Agrobacterium-mediated genetic transformation in recalcitrant rice genotypes is a major limiting factor. This protocol describes a detailed procedure for genome editing with CRISPR-Cas9 in recalcitrant rice genotypes that otherwise show a poor response to tissue culture. With this method, high transformation efficiency can be achieved in relatively a short period.

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Agrobacterium-Mediated Genetic Transformation and Genome Editing Using CRISPR-Cas9 Constructs in Rice

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