Methods in molecular biology (Clifton, N.J.)Oct 1, 2025

Using Agrobacterium and CRISPR-Cas9 to Genetically Modify and Edit Rice

CRISPR Gene Editing Weekly Brief ↗PubMed ↗DOI ↗

Updated Jan 3, 2026

Abstract

High transformation efficiency can be achieved in recalcitrant rice genotypes using CRISPR-Cas9.

CRISPR-Cas9 is an essential tool for creating precise genetic changes in plants.
Recalcitrant rice genotypes typically show poor response to traditional genetic transformation methods.
This protocol provides a detailed procedure to improve genetic transformation in these challenging rice varieties.
The method is designed to expedite the genome editing process in rice.

Simplified

Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas)9 has become an essential tool in every molecular plant breeding laboratory. CRISPR-Cas9 causes highly precise knock-out mutations in plants that can be exploited in crop improvement programmes. However, poor response to Agrobacterium-mediated genetic transformation in recalcitrant rice genotypes is a major limiting factor. This protocol describes a detailed procedure for genome editing with CRISPR-Cas9 in recalcitrant rice genotypes that otherwise show a poor response to tissue culture. With this method, high transformation efficiency can be achieved in relatively a short period.

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Using Agrobacterium and CRISPR-Cas9 to Genetically Modify and Edit Rice

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You found one interesting study. We’ll send the next 7.

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