CRISPR/Cas9-Based Gene Editing in Soybean

Oct 1, 2025Methods in molecular biology (Clifton, N.J.)

Using CRISPR/Cas9 to Edit Genes in Soybeans

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Abstract

A detailed method for CRISPR/Cas9-based gene editing in soybean is provided.

CRISPR/Cas9 is identified as an effective tool for gene editing in crops, particularly soybean.
Mutants have been successfully generated in soybean using the CRISPR/Cas9 system.
The study outlines specific procedures for constructing a CRISPR/Cas9 plasmid for soybean.
Modified protocols for transforming soybean using Agrobacterium are described.
Regeneration techniques from cotyledonary node explants containing Cas9/sgRNA transgenes are detailed.

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CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR-associated Cas9)-based gene editing is a robust tool for functional genomics research and breeding programs in various crops. In soybean (Glycine max), a number of laboratories have obtained mutants by the CRISPR/Cas9 system; however, there has not yet been a detailed method for the CRISPR/Cas9-based gene editing in soybean. Here, we describe the procedures for constructing the CRISPR/Cas9 plasmid suitable for soybean gene editing and the modified protocols for Agrobacterium-mediated soybean transformation and regeneration from cotyledonary node explants containing the Cas9/sgRNA (single-guide RNA) transgenes.

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CRISPR/Cas9-Based Gene Editing in Soybean

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