Identification, cloning, characterization and recombinant expression of an anti-lipopolysaccharide factor from the hemocytes of Indian mud crab, Scylla serrata

The full-length cDNA sequence of Scylla serrata anti-lipopolysaccharide factor (SsALF) is 607 bp and encodes a 97 amino acid protein.

• SsALF protein shows up to 92% similarity with the anti-lipopolysaccharide factor from Scylla paramamosain.
• The SsALF protein contains two highly conserved cysteine residues and a potential lipopolysaccharide binding domain.
• In vivo expression studies indicate that SsALF mRNA is primarily found in hemocytes, heart, and muscle of healthy mud crabs.
• Recombinant SsALF protein exhibits antimicrobial activity against both Gram-positive and Gram-negative bacteria.
• The recombinant protein can significantly neutralize LPS-induced expression of SsALF in vivo, as shown by real-time PCR.
• SsALF is associated with the ability to permeabilize artificial phospholipid membranes, indicating its potential role in microbial defense.

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