Antilipopolysaccharide factor (ALF) of mud crab Scylla paramamosain: Molecular cloning, genomic organization and the antimicrobial activity of its synthetic LPS binding domain

A full-length cDNA of 614 bp encoding an antilipopolysaccharide factor peptide (ALFSp) from the mud crab, Scylla paramamosain, was isolated.

• The ALFSp peptide is composed of 123 amino acids with a calculated molecular mass of 11.18 kDa.
• High identity was found between ALFSp and other known ALFs, with similarities ranging from 59% to 65% among various species.
• The ALFSp gene consists of 1075 bp with three exons and two introns.
• Tissue distribution analysis showed that ALFSp is highly expressed in hemocytes, intestine, and muscle, but not in eyestalk.
• The synthetic ALFSp peptide demonstrated strong antimicrobial activity against Gram-positive bacteria, Micrococcus luteus, and Gram-negative bacteria, Vibrio harveyi.

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