ApoA-IV modulates the secretory trafficking of apoB and the size of triglyceride-rich lipoproteins

Expression of native apoA-IV resulted in a 20-35% increase in triglyceride secretion.

• ApoA-IV modified with the endoplasmic reticulum retention signal KDEL significantly decreased both the rate and efficiency of endogenous apoB secretion.
• Native apoA-IV expression delayed the initial rate of apoB secretion and reduced final secretion efficiency by approximately 40%.
• While apoA-IV-KDEL reduced triglyceride secretion by 75%, native apoA-IV increased triglyceride secretion by 20-35%.
• There was a ∼55% increase in VLDL-associated apoB with native apoA-IV expression.
• Native apoA-IV expression also resulted in a 10.1 nm increase in peak VLDL(1) particle diameter.

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