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Editing Properties of Base Editors with SpCas9-NG in Discarded Human Tripronuclear Zygotes
Gene editing activity of SpCas9-NG base editors in discarded human embryos with three pronuclei
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Abstract
Adenine and cytosine base editors with SpCas9-NG can target DNA sequences with relaxed PAM requirements.
- Various CRISPR-Cas systems, including xCas9, Cas9-NG, and Cas9-SpRY, have expanded the range of targetable DNA sequences for genome editing.
- Editing properties of base editors were analyzed at endogenous sites with NGN protospacer adjacent motifs.
- Human genetic disease-associated DNA point mutations were successfully installed at single and dual sites using base editors with SpCas9-NG.
- The editing properties of base editors were also characterized in discarded human tripronuclear embryos.
- Results suggest that base editors with relaxed PAM requirements may have potential applications in human genetic disease-related research and treatment.
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