Modified Cas9 proteins expand the targeting range of base editors

Six optimized adenine base editors (ABEmax variants) have been engineered to target non-NGG protospacer adjacent motifs.

• The new base editors use SpCas9 variants that are compatible with a broader range of target sequences.
• Circularly permuted Cas9 variants were utilized to create four cytosine and four adenine base editors.
• The editing window of these base editors has been expanded from approximately 4-5 nucleotides to about 8-9 nucleotides.
• These advancements may lead to reduced byproduct formation during the editing process.
• The set of engineered base editors is associated with an improved targeting scope for cytosine and adenine base editing.

Simplified