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Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors
Modified Cas9 proteins expand the targeting range of base editors
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Abstract
Six optimized adenine base editors (ABEmax variants) have been engineered to target non-NGG protospacer adjacent motifs.
- The new base editors use SpCas9 variants that are compatible with a broader range of target sequences.
- Circularly permuted Cas9 variants were utilized to create four cytosine and four adenine base editors.
- The editing window of these base editors has been expanded from approximately 4-5 nucleotides to about 8-9 nucleotides.
- These advancements may lead to reduced byproduct formation during the editing process.
- The set of engineered base editors is associated with an improved targeting scope for cytosine and adenine base editing.
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