Two-Layer Silk-Based Scaffold with Infection-Fighting and Bone-Healing Properties for Treating Infected Bone Injuries

A bilayer hydrogel scaffold (DSHM) was developed with a porous layer approximately 432.1 μm thick for enhanced mechanical strength and a dense layer around 34.6 μm thick for sustained antibacterial effects.

• The scaffold integrates methacrylated silk fibroin with hydroxyapatite and minocycline hydrochloride.
• The porous layer is designed to support bone cell growth and differentiation.
• The dense layer serves as a barrier to prevent fibrous tissue from invading the scaffold.
• The DSHM scaffold shows improved mechanical properties and biocompatibility.
• It may effectively promote bone tissue regeneration while controlling infection.

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