Studying Daily Biological Clocks Using DNA-Protein Interaction Methods

Daily physiological and behavioral changes are associated with rhythmic gene expression driven by a core clock machinery.

• Circadian rhythms involve daily fluctuations in physiology and behavior to maintain homeostasis.
• Transcriptional levels exhibit cyclic changes that contribute to rhythmic gene expression.
• The core clock machinery operates through a transcriptional-translational feedback loop to regulate circadian functions.
• Clock and non-clock transcription factors, along with co-regulators and chromatin modifications, shape the circadian epigenome.
• Cyclic alterations of chromatin structures and molecular functions occur in a coordinated manner during these processes.
• Advancements in high-throughput sequencing technology are enhancing the understanding of circadian research.

Simplified