Chronotherapy to reinforce circadian rhythms improves poststroke outcomes and glymphatic function in mice

Jun 15, 2026The Journal of clinical investigation

Using timed daily treatments to strengthen body clocks improves recovery and brain waste clearance after stroke in mice

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Abstract

Treatment initiated 3 days after stroke significantly improved motor outcomes and reduced brain lesion volume in mice.

  • The small-molecule clock modulator KL001 and high-dose melatonin enhanced glymphatic function in mice.
  • Acute light pulses and active-phase time-restricted feeding also improved glymphatic function.
  • Initiating KL001 or time-restricted feeding after stroke led to increased glymphatic flow.
  • Poststroke brain cytokine levels were lowered with these interventions, indicating reduced inflammation.
  • Reinforcing normal daily rhythms could enhance neurological recovery following a stroke.

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