Method to study how mice anticipate feeding times using an automated feeding device

Food-anticipatory nose poking in mice is a unique behavior associated with the food-entrainable oscillator.

• This behavior occurs under conditions of temporally restricted feeding followed by food deprivation.
• The protocol outlines steps for training and habituating mice to the feeding device.
• Data generation involves creating ethograms from the nose poking behavior recorded by the device.
• The findings may indicate a complex interaction between feeding schedules and the biological clock.

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