Clock gene expression in purified mouse hematopoietic stem cells

Relative differences in clock gene activity were observed in sorted hematopoietic stem cells compared to whole bone marrow.

• Six clock genes, including mPer1 and mCry1, were expressed in mouse hematopoietic stem cells.
• Significant differences in clock gene expression were noted between sorted hematopoietic stem cells and whole bone marrow.
• Clock gene expression and cell cloning ability were unaffected by the high-speed sorting procedure, even after a 24-hour delay.
• The methodology combining high-speed sorting and quantitative PCR is reliable for analyzing clock genes in small stem cell populations.

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