Comparative proteome analysis of the hepatopancreas from the Pacific white shrimp Litopenaeus vannamei under long-term low salinity stress

Apr 25, 2017Journal of proteomics

Protein differences in the shrimp's digestive gland under long-term low salt stress

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Abstract

A total of 533 proteins were identified in the hepatopancreas of Litopenaeus vannamei under low salinity stress (3psu) compared to control salinity (25psu).

84 proteins were differentially expressed, with 58 proteins meeting the 1.2-fold cut-off criteria under low salinity stress.
Of the differentially expressed proteins, 26 were found to be up-regulated and 32 down-regulated.
48 of the 58 differentially expressed proteins were annotated in the Uniprot database and associated with 38 metabolic pathways.
Key pathways identified include those related to energy metabolism, signaling, immune response, detoxification, lipid, and protein metabolism.
L. vannamei exhibited increased glycometabolism and a positive response in detoxification pathways, alongside low immunity under low salinity stress.
The involvement of signaling pathways, particularly Wnt signaling, suggests a complex mechanism for salinity adaptation that requires further exploration.

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UNLABELLED: Litopenaeus vannamei is a typical euryhaline decapod model to study the osmoregulation mechanism in crustaceans. The proteomic was undertaken using isobaric tags for relative and absolute quantification together with the reverse phase in high-performance liquid chromatography mass spectrometry to quantitatively identify the proteins differentially expressed in the hepatopancreas under low salinity stress (3psu) compared with the control salinity (25psu). 533 proteins and 84 differentially expressed proteins were identified including 58 proteins with the 1.2-fold cut-off value under chronically low salinity stress. Among these proteins, 26 were up-regulated while 32 were down-regulated. 48 out of 58 differentially expressed proteins were annotated in the Uniprot database and were mapped into 38 pathways by KEGG analysis. These proteins were categorized into the pathways for energy metabolism, signaling, immunization and detoxification, lipid and protein metabolism. A more active glycometabolism, positive response detoxification pathway, immunosuppression and positive osmoregulation were identified in L.vannamei under low salinity stress. This study suggests that under chronically low salinity stress, L. vannamei showed low immunity and high demand for energy especially from glycometabolism. Signaling transfer related pathways, especially the Wnt signaling pathways were involved in the process of salinity adaption, but the in-depth mechanism warrants further investigation.

SIGNIFICANCE: In this study, a comprehensive physiological response was studied using proteomics to reveal the underlying mechanism of adaptation to low salinity in L.vannamei, which was the first report on the proteomic response of crustacean to salinity stress. The extensive proteomic investigation on hepatopancreas under low salinity stress provides a new insight into the adaptive mechanism of this euryhaline crustacean species to low salinity.

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Comparative proteome analysis of the hepatopancreas from the Pacific white shrimp Litopenaeus vannamei under long-term low salinity stress

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