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Abstract
All 5 clinical isolates of multidrug-resistant Klebsiella pneumoniae demonstrated resistance to multiple antibiotics, with minimum inhibitory concentrations (MICs) ranging from 4-128 μg/mL for imipenem.
- Resistance gene analysis showed the presence of blaOXA-48-like and blaCTX-M-15 in all isolates, with blaNDM-1 found in one isolate.
- Two CRISPR-based plasmid systems were designed for potential genome editing applications.
- Apramycin at 50 μg/mL was identified as a suitable selection marker for plasmid construction.
- The pSGKP-AmpR(Pro)-ApmR plasmid was successfully constructed, while cloning of Cas9 and APOBEC constructs faced technical challenges.
- The findings suggest a need for isolate-specific plasmid design and optimization of guide RNA sequences.
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