Sensitive and accurate detection of deoxynivalenol (DON) is crucial for public health. The CRISPR/Cas12a system exhibits high specificity and efficiency in biosensing, but challenges remain in non-nucleic acid detection, such as reliance on multiple reporters for dual-mode signal output and low detection sensitivity without amplification. In this study, leveraging the magnetic and fluorescence quenching properties of FeO/Au/PDA and the fluorescence/catalytic capabilities of UiO-66-NH, we developed a multimodal integrated reporter (FU) as FeO/Au/PDA-ssDNA-UiO-66-NH, enabling dual-mode signal output via a single reporter. In the presence of DON, the DON-Ab-aDNA complex activates CRISPR/Cas12a, which then indiscriminately cleaves the single-stranded DNA in FU, releasing free UiO-66-NH. Consequently, the fluorescence signal of UiO-66-NHis restored while it catalyzes TMB to produce a blue color reaction. The CRISPR/Cas12a-based fluorescence-colorimetric dual-mode biosensor (CrisprFU) achieved a colorimetric limit of detection (LOD) for DON of 2.15 × 10 ng/mL (detection range: 2-100 ng/mL) and a fluorescence LOD of 7.96 × 10 ng/mL (detection range: 0.5-40 ng/mL). Successful application in real samples demonstrated average recovery rates of 97.04%-104.4% for fluorescence detection and 96.4%-101.8% for colorimetric detection, confirming its practical potential. Furthermore, by replacing the recognition antibody, the CrisprFU system can be extended to detect other analytes. 3 4 2 3 4 2 2 2 -3 -4
