CRISPR/CAS9 - КОРОЛЬ ГЕНОМНОГО РЕДАКТИРОВАНИЯ, "Молекулярная биология"

Sep 14, 2017Molekuliarnaia biologiia

CRISPR/Cas9: The Leading Tool for Editing Genes

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CRISPR Gene Editing on OpenScience ↗PubMed ↗DOI ↗

Abstract

CRISPR/Cas9 is recognized as an efficient and accessible tool for genome editing.

The review outlines the fundamental organization and function of Cas nucleases.
Efficiency and specificity of Cas9 are critical factors to evaluate in genome editing.
Selecting appropriate sgRNA is essential for successful editing outcomes.
Artificially designed Cas9 variants may enhance genome editing capabilities.
Homologous recombination and nonhomologous end joining are important processes in DNA editing.

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The discovery of CRISPR/Cas9 brought a hope for having an efficient, reliable, and readily available tool for genome editing. CRISPR/Cas9 is certainly easy to use, while its efficiency and reliability remain the focus of studies. The review describes the general principles of the organization and function of Cas nucleases and a number of important issues to be considered while planning genome editing experiments with CRISPR/Cas9. The issues include evaluation of the efficiency and specificity for Cas9, sgRNA selection, Cas9 variants designed artificially, and use of homologous recombination and nonhomologous end joining in DNA editing.

CRISPR/CAS9 - КОРОЛЬ ГЕНОМНОГО РЕДАКТИРОВАНИЯ, "Молекулярная биология"

Abstract

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Related papers

You found one interesting study. We’ll send the next 7.

what lands in your inbox each week:

Recent issues from the crispr gene editing brief