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Expanding the range of CRISPR/Cas9-directed genome editing in soybean
Increasing the range of CRISPR/Cas9 gene editing in soybean
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Abstract
Three variant enzymes, xCas9, SpCas9-NG, and XNG-Cas9, were successfully used to induce genome editing in soybean.
- xCas9 effectively edited the soybean genome using NGG and KGA PAM sequences.
- SpCas9-NG recognized multiple PAM sites, including NGD, RGC, GAA, and GAT.
- XNG-Cas9 cleaved genomic regions at NGG, GAA, and AGY PAMs.
- High-fidelity variants eSpCas9 and exCas9 improved specificity for the GGA PAM without affecting on-target editing efficiency.
- These findings may broaden the potential for Cas9-mediated genome editing in soybean.
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