Gene Editing Using CRISPR-Cpf1 Controlled by a Single Two-Way Promoter

A compact adeno-associated virus (AAV) vector enables the co-expression of AsCpf1 and multiple CRISPR RNAs.

• The single bidirectional H1 promoter supported indel formation on par with traditional dual-promoter systems.
• This approach allows for scalable genome editing across single, dual, and triple target configurations.
• Successful genome editing was achieved both in laboratory settings and in living organisms following AAV delivery.
• The engineered AAV vector platform may enhance the feasibility of CRISPR-based therapies.

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