Gene Editing Using CRISPR-Cpf1 Controlled by a Single Two-Way Promoter
Updated
Abstract
A compact adeno-associated virus (AAV) vector enables the co-expression of AsCpf1 and multiple CRISPR RNAs.
- The single bidirectional H1 promoter supported indel formation on par with traditional dual-promoter systems.
- This approach allows for scalable genome editing across single, dual, and triple target configurations.
- Successful genome editing was achieved both in laboratory settings and in living organisms following AAV delivery.
- The engineered AAV vector platform may enhance the feasibility of CRISPR-based therapies.
Simplified
Funding
Competing interests
Y.H.S. and G.-N.K. have registered a patent titled, “Single Adeno-associated virus (AAV) vector expressing CRISPR-Cpf1 using a bidirectional H1 promoter,” to the Korean Intellectual Property Office. The authors declare no conflicts of interest.
PubMed