Effects of high-fat diet on plasma lipids, adiposity, and inflammatory markers in ovariectomized C57BL/6 mice

Nov 15, 2011Nutrition (Burbank, Los Angeles County, Calif.)

High-fat diet’s impact on blood fats, body fat, and inflammation in female mice without ovaries

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Abstract

OVX mice fed a high-fat diet gained about 52% more weight compared to those on a standard chow diet.

OVX mice exhibited atrophied uterus, confirming successful surgical menopause.
Despite consuming less food, OVX-HF mice had 372% more ovarian fat pad compared to OVX-SC mice.
Biochemical analysis revealed OVX-HF mice had 51% higher total cholesterol and 158% higher triglycerides than OVX-SC mice.
Serum adiponectin levels were 40% lower and plasma leptin levels were 323% higher in OVX-HF mice compared to OVX-SC mice.
OVX-HF mice showed increased IL-6 levels, being 241% higher than SHAM-HF and 870% higher than OVX-SC mice.

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OBJECTIVE: We hypothesized that a high-fat (HF) diet aggravates ovariectomy-related complications. To test this hypothesis, ovariectomized (OVX) mice were fed a HF diet, and we investigated the lipid metabolism, adipose tissue remodeling, adipokines, and inflammatory cytokines.

METHODS: To investigate the situation in a mouse model of ovariectomy, OVX and SHAM C57BL/6 mice fed a HF diet (60% fat) or standard chow (SC, 10% fat) were monitored for 18 wk. We evaluated daily food intake and weekly body weight. Mice were killed at 30 wk of age. Blood samples and adipose tissue were collected for biochemical, histologic, and molecular analysis.

RESULTS: OVX groups showed atrophied uterus compared to the SHAM groups, ensuring the success of surgically induced menopause. Despite lower food intake, OVX-HF mice gained about 52% more weight and had heavier total body fats, especially in relation to ovarian fat pad (372%)-a visceral fat which is associated with increased pathogenicity in obesity, and showed larger adipocytes (30%) when compared to OVX-SC mice. Biochemical analysis showed that the OVX-HF mice had increased levels of serum total cholesterol (51%), greater serum triglycerides (158%), lower serum adiponectin (40%), and higher plasma leptin (323%) than OVX-SC mice. The obese group (OVX-HF) also had higher IL-6 levels than both SHAM-HF (241%) and OVX-SC mice (870%).

CONCLUSION: OVX C57BL/6 mice fed HF diet had greater adipose fat pad, larger adipocytes, and increased inflammatory markers, reinforcing the idea that a HF diet aggravates the complications of ovariectomy-associated inflammation.

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Effects of high-fat diet on plasma lipids, adiposity, and inflammatory markers in ovariectomized C57BL/6 mice

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