Antiobesity activities of indole-3-carbinol in high-fat-diet–induced obese mice

Mar 12, 2011Nutrition (Burbank, Los Angeles County, Calif.)

Indole-3-carbinol's anti-obesity effects in mice fed a high-fat diet

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Abstract

Indole-3-carbinol (I3C) treatment resulted in significant reductions in body weight and epididymal adipose tissue weight in high-fat-diet-induced obese mice.

Body weight and epididymal adipose tissue weight were significantly greater in high-fat diet mice compared to the basal and I3C-treated groups.
I3C treatment improved glucose tolerance and increased serum adiponectin levels while decreasing serum glucose, triacylglycerol, insulin, and leptin concentrations.
I3C treatment led to reduced macrophage accumulation and expression of F4/80 in epididymal adipose tissue.
The expression of acetyl coenzyme A carboxylase mRNA was decreased and peroxisome proliferator-activated receptor-γ protein expression was increased in the adipose tissue of I3C-treated mice.

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OBJECTIVE: This study investigated the effects of indole-3-carbinol (I3C), a cruciferous vegetable derivative, on obesity and its associated factors in high-fat-diet-induced obese (DIO) mice.

METHODS: Eighteen male C57BL/6 mice were randomly assigned to one of three groups: basal, high fat (HF), and HF + 5 mg/kg of I3C intraperitoneally (HFI). After 12 wk of treatment, obesity-associated factors, including body weight, organ weight, serum concentrations of glucose, triacylglycerol, insulin, and adipokines, and macrophage accumulation and lipid metabolism-associated factors in epididymal adipose tissue were measured.

RESULTS: Body weight and epididymal adipose tissue weight were greater (P < 0.01), and adipocytes were larger in the HF group than in the basal and HFI groups. Compared with the HF group, the HFI group had improved glucose tolerance, a higher serum adiponectin concentration, lower serum glucose, triacylglycerol, insulin, and leptin concentrations, and less F4/80 expression in epididymal adipose tissue (P < 0.001). Furthermore, I3C treatment decreased acetyl coenzyme A carboxylase mRNA expression (P < 0.05) and increased peroxisome proliferator-activated receptor-γ protein expression (P < 0.05) in epididymal adipose tissue of DIO mice.

CONCLUSION: The I3C treatment decreased body weight and fat accumulation and infiltrated macrophages in epididymal adipose tissue of DIO mice, and these reductions were associated with improved glucose tolerance and with modulated expression of adipokines and lipogenic-associated gene products, including acetyl coenzyme A carboxylase and peroxisome proliferator-activated receptor-γ.

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Antiobesity activities of indole-3-carbinol in high-fat-diet–induced obese mice

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