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Improving Electrochemical Early Detection of African Swine Fever Using CRISPR-Activated DNA Nanostructures
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Abstract
The detection limit of the DNF-amplified biosensor is 3.57 aM, significantly lower than the conventional method's 2.90 fM.
- An electrochemical biosensor was developed for detecting African swine fever virus DNA using CRISPR technology.
- Porous DNA nanoflowers were created to enhance sensitivity and reduce detection time.
- In the presence of ASFV DNA, the Cas12a enzyme was activated, leading to a measurable electrochemical signal change.
- The biosensor demonstrated strong reproducibility, stability, and practical application potential for early diagnosis of ASF.
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