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Abstract
The ECL-RET biosensor demonstrates a detection limit as low as 3.0 fM for circulating tumor DNA (ctDNA).
- The biosensor utilizes Ru(phen) as an energy donor embedded in double-stranded DNA and Au nanocages as the energy acceptor.
- It is based on the target-activated CRISPR-Cas12a system, which enhances the efficiency of resonance energy transfer.
- The detection range of the biosensor spans from 10 fM to 1 nM for ctDNA.
- The system effectively recognizes the L858R mutation in ctDNA from non-small cell lung cancer patients.
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