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Development of formaldehyde dehydrogenase-coupled assay and antibody-based assays for ALKBH5 activity evaluation
Tests to measure ALKBH5 enzyme activity using formaldehyde detection and antibodies
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Abstract
Two assays for evaluating ALKBH5 activity were developed, achieving Z' factors of 0.74.
- N6-methyladenosine is the most common internal modification found in eukaryotic messenger RNA.
- ALKBH5 is a demethylase that influences mRNA export and RNA metabolism.
- Previous attempts to identify modulators of ALKBH5 were hindered by a lack of suitable in vitro assays.
- The newly developed assays utilize different principles to assess ALKBH5 activity.
- Both assays are designed for high-throughput screening in a 384-well plate format.
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