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Functional Significance of FRH in Regulating the Phosphorylation and Stability of Neurospora Circadian Clock Protein FRQ
Role of FRH in controlling the modification and stability of a key clock protein in Neurospora
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Abstract
The FRQ-FRH complex formation is essential for clock function in Neurospora, affecting protein interactions and stability.
- Mapping identified the minimal FRQ region necessary for its interaction with FRH.
- The FRQ-FRH complex is required for the interaction between FRQ and the White Collar Complex (WCC).
- FRQ is also necessary for the FRH-WCC interaction within the FRQ-FRH complex.
- Disruption of FRQ-FRH interaction leads to hypophosphorylation and rapid degradation of FRQ.
- Down-regulation of FRH results in low levels of WHITE COLLAR-1 and WHITE COLLAR-2.
- Rapid FRQ degradation without FRH occurs through a pathway independent of the FWD-1 ubiquitin E3 ligase.
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