Role of FRH in controlling the modification and stability of a key clock protein in Neurospora

The FRQ-FRH complex formation is essential for clock function in Neurospora, affecting protein interactions and stability.

• Mapping identified the minimal FRQ region necessary for its interaction with FRH.
• The FRQ-FRH complex is required for the interaction between FRQ and the White Collar Complex (WCC).
• FRQ is also necessary for the FRH-WCC interaction within the FRQ-FRH complex.
• Disruption of FRQ-FRH interaction leads to hypophosphorylation and rapid degradation of FRQ.
• Down-regulation of FRH results in low levels of WHITE COLLAR-1 and WHITE COLLAR-2.
• Rapid FRQ degradation without FRH occurs through a pathway independent of the FWD-1 ubiquitin E3 ligase.

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