Gene Expression Profiling of the Cephalothorax and Eyestalk in Penaeus Monodon during Ovarian Maturation

Feb 23, 2012International journal of biological sciences

Gene activity in the head and eye stalk of Penaeus monodon during egg development

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Abstract

Numerous transcripts associated with ovarian maturation were isolated from wild-caught and captive-reared prawns.

Gene transcripts were found to be differentially expressed during ovarian maturation in both the cephalothorax and eyestalk.
In the cephalothorax, notable differentially expressed genes included those coding for proteins involved in lipid binding and energy metabolism.
Transcripts in the eyestalk were identified that are linked to receptor activity modulation and transcription regulation.
No differentially expressed transcripts encoding known reproductive neuropeptides were identified in the eyestalk across ovarian maturation stages.
The absence of identified neuropeptide transcripts may relate to their low expression levels in the studied prawns.

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In crustaceans, a range of physiological processes involved in ovarian maturation occurs in organs of the cephalothorax including the hepatopancrease, mandibular and Y-organ. Additionally, reproduction is regulated by neuropeptide hormones and other proteins released from secretory sites within the eyestalk. Reproductive dysfunction in captive-reared prawns, Penaeus monodon, is believed to be due to deficiencies in these factors. In this study, we investigated the expression of gene transcripts in the cephalothorax and eyestalk from wild-caught and captive-reared animals throughout ovarian maturation using custom oligonucleotide microarray screening. We have isolated numerous transcripts that appear to be differentially expressed throughout ovarian maturation and between wild-caught and captive-reared animals. In the cephalothorax, differentially expressed genes included the 1,3-β-D-glucan-binding high-density lipoprotein, 2/3-oxoacyl-CoA thiolase and vitellogenin. In the eyestalk, these include gene transcripts that encode a protein that modulates G-protein coupled receptor activity and another that encodes an architectural transcription factor. Each may regulate the expression of reproductive neuropeptides, such as the crustacean hyperglycaemic hormone and molt-inhibiting hormone. We could not identify differentially expressed transcripts encoding known reproductive neuropeptides in the eyestalk of either wild-caught or captive-reared prawns at any ovarian maturation stage, however, this result may be attributed to low relative expression levels of these transcripts. In summary, this study provides a foundation for the study of target genes involved in regulating penaeid reproduction.

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Gene Expression Profiling of the Cephalothorax and Eyestalk in Penaeus Monodon during Ovarian Maturation

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