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Induction of Ovarian Maturation and Spawning in Penaeus monodon Broodstock by Double-Stranded RNA
Using double-stranded RNA to trigger egg development and spawning in black tiger shrimp
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Abstract
A single injection of 3 µg GIH-dsRNA per gram body weight can inhibit GIH expression for at least 30 days.
- Silencing of GIH expression through GIH-dsRNA is linked to increased vitellogenin levels in female P. monodon.
- This method led to ovarian maturation and spawning in both domesticated and wild female shrimp.
- The effects of GIH-dsRNA on ovarian maturation are comparable to those achieved by traditional eyestalk ablation.
- This approach may offer a less damaging alternative to the practice of eyestalk ablation for inducing spawning.
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