Silencing of Gonad-Inhibiting Hormone Transcripts in Litopenaeus vannamei Females by use of the RNA Interference Technology

Nov 18, 2015Marine biotechnology (New York, N.Y.)

Using RNA Interference to Shut Down Reproductive Hormone Genes in Female Whiteleg Shrimp

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Abstract

A 71% reduction in gonad-inhibiting hormone (GIH) mRNA levels was achieved in shrimp females using RNA interference technology.

  • Gene silencing of GIH was performed in female Litopenaeus vannamei using RNAi technology.
  • Histological analysis revealed only pre-vitellogenesis and primary vitellogenesis oocytes in treated females, while eyestalk-ablated females exhibited all developmental stages, predominantly secondary vitellogenesis and mature oocytes.
  • The anticipated increase in ovary vitellogenin mRNA expression occurred only at 37 days post-injection.
  • This research represents the first application of RNAi technology as an alternative to eyestalk ablation for stimulating gonadal maturation in captive shrimp.

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