Regulating gonad inhibition and vitellogenin/vitellin induction in Penaeus monodon using mature GIH fusion protein and polyclonal antisera

Nov 7, 2016Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Controlling reproductive hormone suppression and egg protein production in Penaeus monodon using mature hormone protein and antibodies

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Abstract

The mature GIH protein (mf-PmGIH) of Penaeus monodon produced a 31.55% reduction in vitellogenin/vitellin levels within 72 hours after injection into juvenile shrimp.

  • GIH plays a critical role in regulating gonad development and reproduction in shrimp.
  • The gene for mature GIH, consisting of 237 base pairs encoding 79 amino acids, was successfully cloned and expressed in E. coli.
  • The expression vector produced a 32.16 kDa thioredoxin-fused GIH protein.
  • Polyclonal antiserum against mf-PmGIH was generated and demonstrated an increase of 74.10% in vitellogenin/vitellin levels within 6 hours after injection.
  • The immunolocalization of anti-mf-PmGIH revealed GIH-producing neurosecretory cells in the eyestalk of P. monodon.

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