Induction of Ovarian Maturation in Penaeus monodon by Molecular Signal Interventional Approach

VTG mRNA levels increased in the ovary and hepatopancreas of unilateral eyestalk-ablated and inhibitor-treated shrimps.

• Vitellogenin (VTG) synthesis is negatively regulated by vitellogenesis-inhibiting hormone (VIH) in penaeid shrimp.
• Eyestalk ablation is commonly used to induce ovarian maturation but can lead to loss of the spawner.
• Inhibition of the MEK pathway using U0126 resulted in no detection of phospho-MEK in treated shrimps.
• DAX-1, SF-1, and StAR gene expression changes were associated with alterations in VIH mRNA and phospho-ERK levels.
• The findings suggest that suppression of DAX-1 may lead to increased SF-1 and StAR protein levels, which are involved in estradiol production linked to vitellogenesis.
• This study provides new insights into the molecular mechanisms of VIH suppression, offering a potentially less-invasive alternative to traditional methods.

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