Genome editing in plants with MAD7 nuclease

The CRISPR-MAD7 system achieved up to 65.6% efficiency in generating regenerated mutant rice and wheat plants.

• MAD7 is a newly engineered nuclease from the Class 2 type V-A CRISPR-Cas family, with low similarity to traditional Cas12a nucleases.
• It recognizes specific DNA sequences known as T-rich PAM sequences (YTTN) in plants.
• Editing efficiency of the CRISPR-MAD7 system in rice and wheat is comparable to the established CRISPR-LbCas12a system.
• Two variants, MAD7-RR and MAD7-RVR, expand the target range of the MAD7 nuclease.
• The M-AFID system enables the creation of predictable DNA deletions at specific sites.
• MAD7 supports multiplex gene editing and can generate insertions or deletions when used with other CRISPR RNA variants.

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