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Using ErCas12a CRISPR-MAD7 to Create Genetic Models in Human Cells, Mice, and Rats

CRISPR Gene Editing Weekly Brief ↗PubMed ↗DOI ↗OA ↗

Updated

Abstract

MAD7 can generate live-born transgenic animals through targeted gene editing in mouse and rat embryos.

  • MAD7 is an engineered system that demonstrates gene editing activity in human cancer cell lines.
  • It generates and allows for fluorescent tagging of endogenous genes in HCT116 and U2OS cells.
  • In mouse and rat embryos, MAD7 is proficient in creating small DNA insertions of 23 bases and larger integrations ranging from 1 to 14 kb.
  • Due to its unique requirements for guide RNA and protospacer adjacent motifs, MAD7 may expand the capabilities of genome engineering.

Simplified

Key numbers

20%
Editing Efficiency in Human Cells
MAD7-induced in HCT116 cells.
60%
Mutation Rate in Mouse Embryos
E14 embryos with mutations at the Rosa26 locus.

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Funding

Competing interests

All of the authors are or were full-time employees of ENVIGO Research Model Services or Horizon Discovery.
PubMed

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