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ErCas12a CRISPR-MAD7 for Model Generation in Human Cells, Mice, and Rats
Using ErCas12a CRISPR-MAD7 to Create Genetic Models in Human Cells, Mice, and Rats
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Abstract
MAD7 can generate live-born transgenic animals through targeted gene editing in mouse and rat embryos.
- MAD7 is an engineered system that demonstrates gene editing activity in human cancer cell lines.
- It generates and allows for fluorescent tagging of endogenous genes in HCT116 and U2OS cells.
- In mouse and rat embryos, MAD7 is proficient in creating small DNA insertions of 23 bases and larger integrations ranging from 1 to 14 kb.
- Due to its unique requirements for guide RNA and protospacer adjacent motifs, MAD7 may expand the capabilities of genome engineering.
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Key numbers
20%
Editing Efficiency in Human Cells
MAD7-induced in HCT116 cells.
60%
Mutation Rate in Mouse Embryos
E14 embryos with mutations at the Rosa26 locus.