Mapping the DNA Targeting Accuracy of dCpf1 Cytidine Base Editors Across the Genome Using Digenome-Seq

Digenome-seq allows for the precise identification of genome-wide targets of programmable nucleases with a focus on dLbCpf1-BE.

• The method enables the detection of C-to-U conversions at both on-target and off-target genomic sites.
• Single-strand breaks are created by removing uracil from the modified DNA, facilitating further analysis.
• Sequencing reads are aligned to a reference genome, providing clear identification of specific cleavage sites.
• The Digenome-seq computer tool is used to identify in vitro cleavage sites based on sequencing data.

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