Green tea polyphenols ameliorate non-alcoholic fatty liver disease through upregulating AMPK activation in high fat fed Zucker fatty rats

Jun 23, 2017World journal of gastroenterology

Green tea compounds improve fatty liver disease by boosting energy regulation in obese rats fed a high-fat diet

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Abstract

GTP treatment resulted in a 31.0% reduction in visceral fat in Zucker fatty rats.

Genetically obese Zucker fatty rats on a high-fat diet exhibited metabolic features similar to human non-alcoholic fatty liver disease (NAFLD).
GTP treatment decreased weight gain by 10.1% and significantly lowered fasting serum insulin, glucose, and lipid levels compared to controls.
Serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were significantly reduced in GTP-treated rats.
GTP treatment attenuated elevated levels of inflammatory cytokines TNFα and IL-6 in circulation.
The intervention reduced hepatic triglyceride accumulation and lipid droplet formation, linked to increased expression of proteins involved in reducing liver fat production.

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AIM: To investigate protective effects and molecular mechanisms of green tea polyphenols (GTP) on non-alcoholic fatty liver disease (NAFLD) in Zucker fatty (ZF) rats.

METHODS: Male ZF rats were fed a high-fat diet (HFD) for 2 wk then treated with GTP (200 mg/kg) or saline (5 mL/kg) for 8 wk, with Zucker lean rat as their control. At the end of experiment, serum and liver tissue were collected for measurement of metabolic parameters, alanine aminotransferase (ALT) and aspartate aminotransferase (AST), inflammatory cytokines and hepatic triglyceride and liver histology. Immunoblotting was used to detect phosphorylation of AMP-activated protein kinase (AMPK) acetyl-CoA carboxylase (ACC), and sterol regulatory element-binding protein 1c (SREBP1c).

RESULTS: Genetically obese ZF rats on a HFD presented with metabolic features of hepatic pathological changes comparable to human with NAFLD. GTP intervention decreased weight gain (10.1%,= 0.052) and significantly lowered visceral fat (31.0%,< 0.01). Compared with ZF-controls, GTP treatment significantly reduced fasting serum insulin, glucose and lipids levels. Reduction in serum ALT and AST levels (both< 0.01) were observed in GTP-treated ZF rats. GTP treatment also attenuated the elevated TNFα and IL-6 in the circulation. The increased hepatic TG accumulation and cytoplasmic lipid droplet were attenuated by GTP treatment, associated with significantly increased expression of AMPK-Thr172 (< 0.05) and phosphorylated ACC and SREBP1c (both< 0.05), indicating diminished hepatic lipogenesis and triglycerides out flux from liver in GTP treated rats. P P P P P

CONCLUSION: The protective effects of GTP against HFD-induced NAFLD in genetically obese ZF rats are positively correlated to reduction in hepatic lipogenesis through upregulating the AMPK pathway.

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Green tea polyphenols ameliorate non-alcoholic fatty liver disease through upregulating AMPK activation in high fat fed Zucker fatty rats

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